Potential answers for "Queen's subject"

⭐⭐⭐⭐ BEE
⭐⭐⭐⭐ ANT
⭐⭐⭐⭐ HONEYBEE
⭐⭐ DRONE
⭐⭐ SHE

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Verdi's penultimate major work   Bond, e.g.   Acts immediately