LIGATION

Ligation

Ligation in molecular biology is the joining of two nucleic acid fragments through the action of an enzyme. It is an essential laboratory procedure in the molecular cloning of DNA whereby DNA fragments are joined together to create recombinant DNA molecules, such as when a foreign DNA fragment is inserted into a plasmid. The ends of DNA fragments are joined together by the formation of phosphodiester bonds between the 3'-hyroxyl of one DNA termini with the 5'-phosphoryl of another. RNA may also be ligated similarly. A co-factor is generally involved in the reaction, and this is usually ATP or NAD+.

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ligation

Noun

  1. Something that ties, a ligature.
  2. The act of tying, of applying a ligature.
  3. The state of having a ligature, of being tied.
  4. The act of tying off or sealing a blood vessel or fallopian tube during surgery.
  5. The formation of a complex by reaction with a ligand


The above text is a snippet from Wiktionary: ligation
and as such is available under the Creative Commons Attribution/Share-Alike License.

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